Dynamic cell seeding allows higher cell seeding efficacy to engineered 3D scaffolds therefore it is mostly preffered by the scientists. Fibroblasts tend to attach on the silicone tube wall but this stiuation is not appropriate for reching maximum cell seeding efficacy on a scaffold. Preventing cells for not to attach on silicone tube, applied force on a single fibroblast should exceed 6500 picoNewton (required minimum force). In the designed cell culture dynamic fibroblast seeding experiment, silicone tube inner diameter is 3.8 mm and fibroblasts surface area is 450 μm2. Please calculate the minimum flow rate as liter per minute of the fibroblasts included cell culture media through silicone tube to prevent fibroblasts adhesion on silicone tubes.