A biochemist has discovered a new membrane protein in a eukaryotic cell. To determine the type of membrane protein, she decides to perform laboratory tests on the cell. She discovers that the protein is an integral membrane protein. Select the experimental scenarios that correctly support the finding that the protein is an integral membrane protein? Try to dissolve the protein out of the membrane with an aqueous buffer, then try to dissolve the protein with a nonpolar solvent. If the protein is solubilized in the nonpolar solvent but not in the aqueous buffer, it is probably an integral protein. Try to dissolve the protein out of the membrane with an aqueous buffer, then try to dissolve the protein with a detergent solution. If the protein is solubilized in the detergent solution but not in the aqueous buffer, it is probably an integral protein. Try to dissolve the protein out of the membrane with an aqueous buffer at low pH, then try to dissolve the protein with an aqueous buffer at neutral pH. If the protein is solubilized in the buffer at low pH but not in the buffer at neutral pH, it is probably an integral protein. Try to dissolve the protein out of the membrane with an aqueous buffer containing a chelating agent that removes Ca²+, then try to dissolve the protein in an aqueous buffer without the chelating agent. If the protein is solubilized in the buffer with the chelating agent but not in the buffer without the chelating agent, it is probably an integral protein. Try to dissolve the protein out of the membrane with an aqueous buffer containing urea, then try to dissolve the protein in an aqueous buffer without urea. If the protein is solubilized in the buffer with urea but not in the buffer without urea, it is probably an integral protein.