Question 3 A Amylase is a protein that catalyzes the conversion of starch to simple sugars. Amylase activity in an aqueous solution can be measured by using iodine as a starch indicator. A solution containing iodine and starch will have a dark blue color, whereas a solution containing iodine but no starch will have a light brown color. The color change of an iodine solution from dark blue to light brown can be used to measure the rate at which starch is converted to simple sugars. A student designs an experiment to investigate the effect of environmental pH on amylase function. The design of the experiment is presented in Table 1. Which of the following statements best justifies the inclusion of test tube V as a control in the experiment? (A) It will provide a measurement of amylase activity at an acidic pH. (B) It will provide a measurement of amylase activity at a basic pH. (C) It will show the color change that occurs in the absence of enzyme activity. (D) It will show the color change that occurs in the absence of the amylase protein.