< HOLATION METHODS. QUANTIFICATION BY COLONY COUNTING INTRODUCTION LABORATORY SIMULATION Using the count data and observational data you acquired, calculate the number of CFUs in the original sample, Number of CFUS CFU. You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1ml. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original sample. Number of CFUs - CFUS Lab Data Х Phase 1 Plate # Dilution Label Growth Appearance on Plate Isolated Colonies Present? Countable? 1 1:10,000 Heavy No Too numerous to count 2 1:100,000 Yes, few Too numerous to count Heavy Intermediate 3 Yes, many Countable 1:1,000,000 1:10,000,000 4 Scant Yes, few Too few to count OS.COM coopoopia Image Plate 1 plate 2 Plate 3 Plate + Phase 2 Plake 3 1 Plate Selected For Counting Volume of Diluted Sample (mL) Sample Dilution Colony Count 1:1,000,000 54 How to calculate Bacterial concentration in Original Sample