expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of mrna and reverse transcriptase is part of a strategy to solve the problem of expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of and reverse transcriptase is part of a strategy to solve the problem of restriction fragment ligation. post-transcriptional processing. nucleic acid hybridization. post-translational processing.