6) In a recombinant frequency gene mapping experiment, Hfr StrS a+b+c- cells were mated with F- cells of the genotype StrR a-b- c+. Exconjugates were selected on media lacking A (compound required for growth of a- cells) and the genotypes of 1000 colonies that grew on A deficient media were determined with appropriate selective media as indicated below (show work).
Genotype Number of colonies
a+b- c+ 395
a+b- c-
a+b+c+10
a+b+c- 190
a) What is the late marker? b) What is the gene order?
c) What are the map distances between the 3 markers?
