Record the sequence of non-mutated DNA:
G A T C G A T A C C A T T C G G C G C A T A C T T C G
Using this sequence of DNA, make a substitution mutation; choose any base in the sequence and randomly change it to another base.
For example: You could choose a C and make it a G. Record the new DNA sequence and underline the changed base.
Transcribe the mutated DNA sequence.
Translate the mutated DNA sequence; begin at the start codon and end at the stop codon.
Note: Begin translation at the first start codon, AUG, that you see when reading the mRNA sequence from left to right. Stop translating the sequence when you reach the first stop codon in the reading frame.
Describe the effect of the substitution mutation on the sequence of amino acids that is produced. Reflect on the series of amino acids that was produced under normal conditions.