Answer :
When Cas9 cuts DNA, when the cell reattaches the cut ends of the DNA back together, it frequently introduces random mutations.
What is the CRISPR-associated endonuclease?
During an adaptive bacterial immune response, Type II CRISPR–Cas (CRISPR-associated) systems generate double-strand breaks in invasive DNA by utilizing an RNA-guided DNA endonuclease known as Cas9.
When DNA fragments are joined together, which enzyme is used?
An enzyme that joins DNA is DNA ligase. Ligase can join two pieces of DNA together to form a single, unbroken molecule of DNA if their ends match.
What happens when DNA is cut by CRISPR?
At the point when the Cas9 complex cuts DNA, it cuts the two strands at similar spot, leaving 'obtuse closures' that frequently go through transformations as they are rejoined. The exposed ends have short overhangs because the Cpf1 complex offsets the cuts in the two strands.
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