Answered

you want to use crispr/cas9 to model a deletion mutation commonly found in cancer cells. suppose you design two sgrnas to this region of the genome. if cas9 cuts dna at 2 loci, what is the approximate size of the dna fragment expected to be deleted? 5'-tattccacgacacagcatgtgctcttat..76bps..acaagagaagtacagtaatgacggactagta-3' 78bp 76 bp you cannot model deletion mutations using 2 sgrnas 124 bp 112bp