transmission electron microscopes that use high-energy electrons accelerated over a range from 40.0 to 100 kv are employed in many applications including the study of biological samples (like a virus) and nanoscience research and development (alloy particles and carbon nanotubes, for example). what would be the spatial limitation (in pm) for this range of electrons? it is often true that resolution is limited by the optics of the lens system, not by the intrinsic limitation due to the de broglie wavelength.