While culturing three different human cell lines (Cell X, Cell Y and Cell Z), you decided to culture some of these cell lines together and observed following phenotypes. 1: When Cell X and Cell Y were cultured together in single dish, Cell X and Cell Y formed isolated cell colonies (clusters of cells) and each colony only contained one type of cells (Cell X or Cell Y). 2: When Cell Y and Cell Z were cultured together in single dish, they form colonies that always had Cell Y at the center of colonies and Cell Z was always on the periphery of the colonies.
Q1) What is the key protein family responsible for these phenotypes? How it is different among Cell X, Cell Y and Cell Z? (4pts)
Q2) If you culture Cell X and Cell Z together, what phenotype would you observe in cell colony formation? Why do you think this would be the case? (4pts)