According to the Michaelis-Menten equation, what is the ratio of V/Vmax when [S] = 15Km? Express the ratio as a decimal rounded to 2 decimal places_ b. (5 pts) According to the Michaelis-Menten equation, if the ratio of V/Vmax is 0.30,what is the value of [SJKu ? Express the ratio as a decimal rounded to 2 decimal places. c-d. (10 pts) An experiment is performed in which the enzyme acetylcholinesterase converts two different substrate molecules_ A and B, to product. The table below shows kinetic data for the enzyme operating on and The first two columns show velocity data at different concentrations of A; the last two columns show velocity data at different concentrations of B_ Note that the bottom row shows the calculated Vmax for A and for B. [A] (uM) V (uM/sec) [B] (uM) V (uWsec) 19 5 33 20 70 12 66 45 135 18 86 65 175 28 110 90 215 48 139 100 228 110 175 130 265 180 190 440 405 220 195 700 443 Vmax 220 Vmax 530 By inspecting the table (no math needed), determine the Km of the enzyme for substrate A in terms of UM: Enter the value of Ku (without unit) in question 8 on the online answer form By inspecting the table (no math needed); determine the Kv of the enzyme for substrate B in terms of UM Enter the value of K (without unit) in question 9 on the online answer form: Assume that for the enzyme, the Kn values of the substrate indicate the binding affinities of the substrates for the active site. Which substrate, or B, has higher_binding affinity for the active site? Select the correct answer from the options in question 10 on the online answer form



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