Answer :
An IS element on the F-plasmid and the identical IS element on the host chromosome can recombine to create Hfrs. Many bacterial chromosomes include multiple IS insertions. Consider the wild-type E.
Because they frequently transfer chromosomal genes, bacteria with an F-plasmid incorporated into the chromosome are referred to as Hfr strains.
A bacterium having a conjugative plasmid (such as the F-factor) incorporated into its chromosomal DNA is known as a high-frequency recombination cell (Hfr cell), sometimes known as a Hfr strain. The incorporation of the plasmid into the cell's chromosome is by homologous recombination. F plasmids, not the complete chromosome, are often transferred to the recipient bacterium during bacterial conjugation. Those bacteria are referred to as F+ strains or donors if they carry the F plasmid. They can create sex pili and transfer plasmids to other bacteria that take them in.
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