Which of the following questions will best direct an investigation of the mechanism of ATP synthase?
What is the source of the inorganic phosphate that is used to generate ATP from ADP?
Is the phosphorylation of ADP by ATP synthase dependent on the formation of a proton gradient?
Can ATP synthase use the energy released by phosphorylation of ADP to pump protons against a concentration gradient?
Can oxidative phosphorylation be uncoupled from the electron transport chain?