4. A scientist determined the rate of an enzyme-catalyzed reaction by measuring the amount of product formed over time. The
following curve was generated from the data collected. What is the most likely explanation for the change in the slope of the line
between 4 and 6 minutes?
Product ( moles)
9
20+
123456
Time (minutes)
A. The enzyme had denatured.
B. A large amount of the substrate had been consumed.
C. An allosteric inhibitor appeared.
D. There was a dramatic change in the pH.