Please answer the following questions based on the titration curve:
1. From the plot, determine the number and types of titratable groups for the unknown amino acid.
2. Determine best you can the experimental pKa values for each titratable group from the plot
3. Determine best you can the molecular weight of your unknown amino acid from data obtained from
your plot.
4. Calculate the isoelectric point of your sample. a) What is the definition of isoelectric point? b)
Where would you find the isoelectric point on a titration curve?
5. What is the identity of your unknown amino acid? Justify your conclusion by comparing the
observed molecular weight and pKa values to those for all of the amino acid